A comparison of reproductive outcome using different sperm selection techniques; density gradient, testicular sperm, PICSI, and MACS for ICSI patients with abnormal DNA fragmentation index
نویسندگان
چکیده
منابع مشابه
Comparison of Sperm Telomere Length between Two Sperm Selection Procedures: Density Gradient Centrifugation and Zeta Potential
Background: Telomeres are particular sequences of DNA located at the end of the eukaryotic chromosomes that are essential for genome integrity. Telomere length in spermatozoa differs among males, as well as spermatozoa. Also, decreased telomere length in spermatozoa of infertile men is associated with the reduction of fertility potential and embryo quality. Density gradient centrifugation (DGC)...
متن کاملVariation of DNA Fragmentation Levels During Density Gradient Sperm Selection for Assisted Reproduction Techniques
Predicting the outcome of in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) is one main goal of the present research on assisted reproduction. To understand whether density gradient centrifugation (DGC), used to select sperm, can affect sperm DNA integrity and impact pregnancy rate (PR), we prospectively evaluated sperm DNA fragmentation (sDF) by TUNEL/PI, before and after DG...
متن کاملP-58: Which Novel Sperm Selection is Suitable for ICSI? MACS-DGC or DGC-MACS
Background: Super-paramagnetic annexin-V conjugated micro-beads are able to eliminate sperm with phosphatidylserine externalization (PSE). PSE is considered as apoptotic sperm. PES positive sperm can be removed from sperm preparations sample to enhance sperm quality for assisted medical procreation. Therefore, the efficiency of this technique over normal sperm processing procedure such as densi...
متن کاملRe: Comparison of Reproductive Outcome in Oligozoospermic Men with High Sperm DNA Fragmentation Undergoing Intracytoplasmic Sperm Injection with Ejaculated and Testicular Sperm.
OBJECTIVE To investigate the effectiveness of intracytoplasmic sperm injection (ICSI) using testicular sperm as a strategy to overcome infertility in men with high sperm DNA fragmentation (SDF). DESIGN Prospective, observational, cohort study. SETTING Private IVF centers. PATIENT(S) A total of 147 couples undergoing IVF-ICSI and day 3 fresh ETs whose male partner has oligozoospermia and h...
متن کاملP-20: Efficiency of Magnetic-Activated Cell Sorting System in Separation of Normal Sperm
Background Sperm preparation before assisted reproductive techniques is very important for obtaining successful fertilization and pregnancy. During ICSI technique, sperms are selected based on viability and morphology. But this selection did not guarantee integrity of sperm DNA. Therefore, novel sperm selection procedures are introduced for selection of sperm with low DNA damage. One of these p...
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ژورنال
عنوان ژورنال: Fertility and Sterility
سال: 2018
ISSN: 0015-0282
DOI: 10.1016/j.fertnstert.2018.07.071